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Prevalence of Bordetella pertussis and Bordetella parapertussis in Samples Submitted for RSV Screening
Paul Walsh MB, BCh, BAO, MSc, Department of Emergency Medicine, Kern Medical Center, Bakersfield, CA
Christina Lim-Overmeyer BS, Research and Development Department, Medical Diagnostic Laboratories, Hamilton, NJ
Lauren Kimmel BS, Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ
Melanie Feola BS, Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ
James Pusavaat BS, Department of Pathology, Kern Medical Center, Bakersfield ,CA
Tuan Anh Nguyen, Department of Emergency Medicine, Kern Medical Center, Bakersfield ,CA
Sam Kuan MB, BCh, BAO, Department of Emergency Medicine, Kern Medical Center, Bakersfield CA.
Kirt Emery MPH, Kern County Department of Public Health
Martin Rosengreen MD, Department of Emergency Medicine, Kern Medical Center, Bakersfield ,CA
Eli Mordechai PhD, Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ
Martin E. Adelson PhD, Research and Development Department, Medical Diagnostic Laboratories, Hamilton ,NJ
ABSTRACT:
Background: The clinical presentation of Bordetella pertussis can overlap with that of respiratory syncytial virus (RSV); however, management differs.
Hypothesis: First, the prevalence of B. pertussis is less than 2% among patients screened for RSV, and second the prevalence of B. parapertussis is also less than 2% among these patients.
Methods: Nasal washings submitted to a clinical laboratory for RSV screening were tested for B. pertussis and B. parapertussis, using species-specific real-time polymerase chain reaction (PCR) assays. These were optimized to target conserved regions within a complement gene and the CarB gene, respectively. A Bordetella spp. genus-specific real-time PCR assay was designed to detect the Bhur gene of B. pertussis, B. parapertussis, and B. bronchiseptica. RSV A and B subtypes were tested by reverse transcription-PCR.
Results: Four hundred and eighty-nine clinical samples were tested. There was insufficient material to complete testing for one B. pertussis, 10 RSV subtype A, and four RSV subtype B assays. Bordetella pertussis was detected in 3/488 (0.6%) (95% CI 0.1% to 1.8%), while B. parapertussis was detected in 5/489 (1.0%) (95% CI 0.3% to 2.4%). Dual infection of B. pertussis with RSV and of B. parapertussis with RSV occurred in two and in three cases respectively. RSV was detected by PCR in 127 (26.5%).
Conclusion: The prevalence of B. pertussis in nasal washings submitted for RSV screening was less than 2%. The prevalence of parapertussis may be higher than 2%. RSV with B. pertussis and RSV with B. parapertussis coinfection do occur.
[WestJEM. 2008;9:135-140.]
KEYWORDS:
Bordetella pertussis, Bordetella parpertussis, respiratory syncytial virus
SUGGESTED CITATION:
Paul Walsh MB, BCh, BAO, MSc; Christina Lim-Overmeyer BS; Lauren Kimmel BS; Melanie Feola BS; James Pusavaat BS; Tuan Anh Nguyen; Sam Kuan MB, BCh, BAO; Kirt Emery MPH; Martin Rosengreen MD; Eli Mordechai PhD; and Martin E. Adelson PhD
(2008)
"Prevalence of Bordetella pertussis and Bordetella parapertussis in Samples Submitted for RSV Screening",
Western Journal of Emergency Medicine:
Vol. 9:
No. 3,
Article 2.
http://repositories.cdlib.org/uciem/westjem/vol9/iss3/art2
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