The contribution of nursery habitat types will be determined using trace element analysis of California halibut otoliths (ear bones). These elements can be viewed as signatures that serve as a record of the environmental conditions experienced by fish over time and which differ across habitat types. Also, statistical analysis of long term CalCOFI data will be analyzed to examine how changes of coastal habitat types over time have affected the larval abundances and distributions of the halibut. To determine the level of connectivity between halibut populations, stable isotope analysis of otolith material (which varies with temperature and indicates latitudinal position over time) will be employed as well as molecular tools. A matrix model, that includes life-history information on halibut from specific habitats, will be created. With sensitivity analysis, this model will describe how halibut standing stocks are affected by coastal habitat alteration. Additionally, a patch isolation model will be employed to examine the effects of disturbance on halibut metapopulations (why connectivity data is needed).

This study will identify key nursery habitat for juvenile California halibut and reveal the sensitivity of halibut standing stocks to perturbations of coastal habitat types. Also, this study will be one of few to incorporate microconstituent analysis, molecular tools, and modeling in order to more fully understand population structure and dynamics within a species.