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Technical Completion Report (University of California Water Resources Center), Project Number W-935.

Download the Paper (298 K, PDF file) - September 29, 2003 Tell a colleague about it. Printing Tips: Select 'print as image' in the Acrobat print dialog if you have trouble printing.

ABSTRACT:
A novel, simple, economical, and environmental friendly tunable immunosorbent-based immunoassay for sensitive and selective determination of atrazine is reported. Tunable immunosorbents consisting of a fusion between an elastin-like polypeptide made up of 77 repeating units of the pentapeptide VPGVG and a single chain Fv of an anti-atrazine antibody was synthesized biologically and purified by temperature-triggered phase transition. A competitive immunoassay based on the competition of atrazine-HRP and atrazine was established with IC50 and lower detection limit of 0.16 and 0.01 ppb, respectively. Excellent recoveries (mean values ranging between 92 % and 104 %) were demonstrated in simulated atrazine-contaminated water samples.

SUGGESTED CITATION:
Wilfred Chen and Ashok Mulchandani, "Tunable immunosorbents for the remediation of atrazine- and simazine-contaminated waters" (September 29, 2003). University of California Water Resources Center. Technical Completion Reports. Paper chen.
http://repositories.cdlib.org/wrc/tcr/chen